Description
1. A Bird's Eye View of the Bacterial Landscape
Natividad Ruiz
2. Visualizing the Bacterial Cell Surface: An Overview
Harald Engelhardt
3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems
Nadine S. Henderson and David G. Thanassi
4. Experimental Manipulation of the Microbial Functional Amyloid Called Curli
Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman
5. Visualization of Gram-Positive Bacterial Pili
Chungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That
6. Single Cell Microfluidic Studies of Bacterial Motility
Adrien Ducret, Olivier Th odoly, and T m Mignot
7. Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule
Susan M. Steenbergen and Eric R. Vimr
8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy
Wei Hu, Renate Lux, and Wenyuan Shi
9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance
Elena B. Tikhonova and Helen I. Zgurskaya
10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System
Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist
11. Pore Formation by T3SS Translocators: Liposome Leakage Assay
Eric Faudry, Caroline Perdu, and Ina Attr e
12. Isolation of Bacterial Type IV Machine Subassemblies
Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie
13. Production and Crystallization of Bacterial Type V Secretion Proteins
Hye-Jeong Yeo
14. Assembly of Bacterial Outer Membrane Proteins
Jan Grijpstra, Martine P. Bos, and Jan Tommassen
15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization
Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent
16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles
Halima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn
17. In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate
Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer
18. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes
Filipe Carvalho, Mar a Graciela Pucciarelli, Francisco Garc a-del Portillo, Didier Cabanes, and Pascale Cossart
19. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis
Anna E. Grzegorzewicz and Mary Jackson
20. Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues
Katleen Denoncin, Valerie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, and Jean-Francois Collet
21. Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response
Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio
22. Isolation of Bacteria Envelope Proteins
Shu Quan, Annie Hiniker, Jean-Francois Collet, and James C.A. Bardwell
23.